Sunday, January 17, 2010

Basic Requirement For Training in Biotechnology

It is our suggestion to the biotech students to check the minimum infrastructure for their quality learning in any institute / training company etc for their project work or summer training in the concert areas of life science.

Basic Infrastructure for Molecular Biology Lab

  • Thermo Cycler : For Amplification of Nucleic Acids
  • Spectrophotometer : For Quality Check of DNA or RNA
  • Colorimeter
  • Gel Documentation System with Gel Analysis Software : Required for analysis of Nucleic Acids
  • Refrigerated High Speed Centrifuge : Isolation of DNA or RNA
  • Ultra High Speed Centrifuge : RNA Isolation
  • Mini Centrifuges
  • Electrophoresis Units : Horizontal & Vertical
  • E-Blotter
  • Power Supply ( with variable voltage & current )
  • Dry Bath
  • Water Bath
  • Sonicator
  • Vortex , Magnetic Stirrer , Gel Dryer , Auto Pipettes etc

Basic Infrastructure for Immunology

  • Good Bio safety Cabinet
  • Immuno Electrophoresis
  • ELISA Reader & Washer
  • Fluorescence / phase contract / Bright field Microscope


Basic Infrastructure for Proteomics or Enzymology or Chromatography Techniques

  • Gel Filtration Chromatography ( Robotic Liquid Handling System is required ) : avoid basic glass columns
  • Ion Exchange Chromatography
  • Affinity Chromatography
  • HPLC
  • Spectrophotometer
  • Digital Analytical Balance
  • Microprocessor based Ph Meter
  • Vacuum Rotary Evaporator with chiller
  • 4 Degree scientific purpose refrigerator
  • Wrist watch Shaking Equipment
  • SDS – PAGE with variable power supply
  • Liquid Nitrogen
  • Hot Air Oven
  • Lyophilizer


Basic Infrastructure for Microbiology

  • Horizontal Laminar Air Flow
  • Vertical Laminar Air Flow
  • Incubator Shaker
  • BOD Incubator
  • CO2 Incubator
  • UV Cabinet
  • Autoclaves
  • Incubators & Ovens
  • Colony Counter
  • Colorimeter
  • Turbidity Meter
  • Microscopes
  • Lyophilizer


Basic Infrastructure for Pharmaceutical Testing Lab

  • HPLC
  • Spectrophotometer
  • Digital Analytical Balance
  • High Precision Ph Meter
  • Dissolved Oxygen Meter
  • Conductivity Meter
  • Potentiometer
  • Polari meter
  • Refractometer
  • Viscometer
  • Tintometer
  • Kheldal Distillation & Digestion Unit
  • Soxhlet Extraction Unit
  • Flame Photometer
  • Auto Karl fisher Titrator
  • Leak Test Apparatus
  • Tablet Dissolution Test Apparatus
  • Tablet Disinteragation Test Apparatus
  • Tablet Hardness Tester
  • Melting Point Apparatus

Basic Requirement For Plant Tissue Culture

  • Media Preparation Room
  • Sterile Room for Incubation
  • Plant Growth Chamber

Basic Questions to be asked by the students for quality training :

1. What cenrifugation systems are available for isolation of DNA or RNA ?
2. Which software is available in your gel doc system for the analysis of DNA ?
3. How we will check the quality of DNA / RNA ?
4. How we will set a program in PCR for specific activity ?
5. Do you have quality variable power pack for electrophoresis ?
6. Which chromatography columns are supposed to be used in purification of proteins ?
7. Is there individual handling for us ?

Note : Without the above mentioned infrastructure , no quality training may be given in the concern area of Life Science.


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